Molecular Biology Laboratory Manual

A hands-on manual with step-by-step protocols for PCR, ligation, bacterial transformation, plasmid preps, RNA extraction, real-time PCR, and western blots. It even includes a nanopore sequencing lab.

David Ng

The theory behind the bench, explained clearly. These notes cover DNA purification, gel electrophoresis, vectors and cloning, restriction enzymes, protein gels, and PCR troubleshooting.

David Ng

The complete lab manual of an applied molecular biology course, built around a real genetic screen in yeast. You follow each step, from DNA digests and transformation to plasmid recovery and sequencing.

MIT OpenCourseWare

A full semester of lab work built around one project: amplify a gene by PCR, clone it, express the protein, and purify it. Each chapter adds background, protocols, and expected results.

Felicia Vulcu, Vivian Leong and Caitlin Mullarkey