Applied Molecular Biology Laboratory Manual

A hands-on manual with step-by-step protocols for PCR, ligation, bacterial transformation, plasmid preps, RNA extraction, real-time PCR, and western blots. It even includes a nanopore sequencing lab.

David Ng

The theory behind the bench, explained clearly. These notes cover DNA purification, gel electrophoresis, vectors and cloning, restriction enzymes, protein gels, and PCR troubleshooting.

David Ng

A practical manual organized around a research project on gene regulation. Short chapters cover primer design, PCR, restriction digests, ligation, transformation, and DNA and RNA extraction.

Peter Oelkers

A full semester of lab work built around one project: amplify a gene by PCR, clone it, express the protein, and purify it. Each chapter adds background, protocols, and expected results.

Felicia Vulcu, Vivian Leong and Caitlin Mullarkey