Molecular Techniques Lecture Notes

A hands-on manual with step-by-step protocols for PCR, ligation, bacterial transformation, plasmid preps, RNA extraction, real-time PCR, and western blots. It even includes a nanopore sequencing lab.

David Ng

The complete lab manual of an applied molecular biology course, built around a real genetic screen in yeast. You follow each step, from DNA digests and transformation to plasmid recovery and sequencing.

MIT OpenCourseWare

A practical manual organized around a research project on gene regulation. Short chapters cover primer design, PCR, restriction digests, ligation, transformation, and DNA and RNA extraction.

Peter Oelkers

A full semester of lab work built around one project: amplify a gene by PCR, clone it, express the protein, and purify it. Each chapter adds background, protocols, and expected results.

Felicia Vulcu, Vivian Leong and Caitlin Mullarkey