Molecular Biology Lab Manual

The theory behind the bench, explained clearly. These notes cover DNA purification, gel electrophoresis, vectors and cloning, restriction enzymes, protein gels, and PCR troubleshooting.

David Ng

The complete lab manual of an applied molecular biology course, built around a real genetic screen in yeast. You follow each step, from DNA digests and transformation to plasmid recovery and sequencing.

MIT OpenCourseWare

A practical manual organized around a research project on gene regulation. Short chapters cover primer design, PCR, restriction digests, ligation, transformation, and DNA and RNA extraction.

Peter Oelkers

A full semester of lab work built around one project: amplify a gene by PCR, clone it, express the protein, and purify it. Each chapter adds background, protocols, and expected results.

Felicia Vulcu, Vivian Leong and Caitlin Mullarkey